OpenCGA uses dependencies from Hortonworks HDP-2.5.0 internally. It has not been tested with other flavours of Hadoop.
Download or pull the version you want to try.
You can build the application from sources executing:
You can customize some configuration parameters adding them to the compilation with
-D<param>=<value>. Some interesting params are:
OPENCGA.INSTALLATION.DIRfor changing the installation directory.
OPENCGA.CLIENT.REST.HOSTThis parameter indicates the address of the REST server. For this tutorial we are going to use a embedded REST server.
OPENCGA.CELLBASE.REST.HOSTto specify the cellbase installation.
OPENCGA.CELLBASE.VERSIONto specify the cellbase version.
OPENCGA.STORAGE.DEFAULT_ENGINEto specify the default storage engine. By default is "mongodb", so we will need to add
--storage-engine hadoopto each command. Compile with
-DOPENCGA.STORAGE.to avoid that.
To see the rest of the configurable parameters, check the default-config profile at the main pom.xml.
For example, to change the default engine and the rest host, execute:
Then copy the application (the content of build folder) into the installation directory, by default and in this tutorial this is /opt/opencga.
- See Download and Installation for more information.
Needless to say, the computer where opencga is installed must have access to the Hadoop cluster.
OpenCGA supports multiple versions of Hadoop. More specifically, it can be compiled against specific distributions of Hadoop.
To select a specific version (see table above) add -P<version_key> to the maven command line.
Supported Hadoop Distributions
|hdp-2.5.0||Hortonworks HDP v2.5.0|
|hdp-2.6.0||Hortonworks HDP v2.6.0|
|hdp-3.0.1||Hortonworks HDP v3.0.1||3.1.1||2.0.0||Experimental|
|emr-5.3.0||Amazon EMR Release 5.3.0||2.7.1||1.1.12|
|emr-5.8.0||Amazon EMR Release 5.8.0||2.7.3||1.3.1|
|cdh-5.13.0||Cloudera CDH v5.13.0||2.6.0||1.2.0|
In order to interact with Hadoop, we need to include the Hadoop configuration files to the classpath of the program. This can be automatic, or manual, depending on the way of accessing to Hadoop.
a) Read hadoop configuration from the server
This mode is for hadoop client nodes (or local installations, or hadoop nodes) where the commands 'hadoop', 'yarn' and 'hbase' are installed, and the client configuration updated. The script
bin/opencga-env.sh will add the configuration files to the java classpath. Nothing else is needed.
In this scenario, you should be able to execute this commands:
note: This mode does not work for executing OpenCGA within a Tomcat server. The configuration needs to be provided manually.
b) Provide hadoop configuration manually
In case of not having the hadoop binaries installed in the node, we need to provide the hadoop client configuration files manually. Take the configuration files from the cluster hadoop, and copy them into the folder
conf/hadoop in the installation directory. This folder is automatically added to the classpath.
The hadoop client configuration files are usually distributed across multiple folders. Make sure that you get at least the general configuration files from hadoop (core-site.xml, hdfs-site.xml, yarn-site.xml, ...) and the specific configuration files from hbase (hbase-site.xml, ...).
The installation directory should look like this:
Configure Apache-Tomcat to connect to Hadoop
To provide the configuration files to the opencga server through Apache-Tomcat, we have to include them as a resource in the context.
Simply, place all the configuration files in one single folder, and add this configuration file to tomcat, replacing the "base" property to the location of the hadoop configuration files. The name of the file must match with the name of the opencga war.
To simplify the installation, we are going to use the embedded server for the REST API.
- See Getting started in 5 min for more info.
Using OpenCGA embedded REST server
In case of not having a Tomcat available, or to simplify test environment installation, we can use the embedded server.
Then, we have to make sure that the
client-configuration.yml points to the embedded server, instead of the tomcat server. By default, this server runs on the port 9090.
Indexing a VCF file
For this testing area, we are going to use a sample VCF data from the Platinum genomes. You can use any other file, but all the examples below use the VCF file platinum-genomes-vcf-NA12877_S1.genome.vcf.gz
You can find other files to load in this link: http://swdev.bioinfo.hpc.cam.ac.uk/downloads/datasets/vcf/platinum_genomes/gz/
Once OpenCGA is installed and running, we need to create a new project and study in catalog, and register our VCF file. You can also download all the files from that link
Once everything is set up, just need to load the files. This command line will create an internal job that will be executed by the catalog daemon.
Optionally, we can use the opencga-analysis.sh command line for a synchronous execution:
For testing purposes, it may be interesting to have an standalone installation of OpenCGA-Storage.You can find another build folder at
opencga/opencga-storage/build/ that contains only the binaries for storage
A simple indexation can be done executing the next command:
Annotate the variants database.
At this point, the last but not least, is annotate the variants. Despite this can be done at the same time than indexing variant files, it may be more clear in separated executions:
This will annotate all the variants without annotation at the database, skipping the already annotated variants.
And we are done! At this point we will be ready to query variants. Here are some examples commands:
- Count number of variants
./opencga.sh variant query --study platinum --count
- Get the first 10 variants from the Chromosome 8
./opencga.sh variant query --study platinum --region 8 --limit 10 --sort
- Count variants in gene BRCA2
./opencga.sh variant query --study platinum --gene BRCA2 --count
You can find the full list of options at the help:
./opencga.sh variant query --help
You can find other query examples at this other tutorial: Querying Variants with the Command Line
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