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OpenCGA needs Java 8 to compile and run, we recommend to use Oracle JDK 1.8.0_60+ since is the one we use and therefore it is fully supported. There are two main ways of installing Oracle JDK in Linux: via package manager and manually.
Installing via package manager can be more or less easy to do depending on the Linux distribution you use, for example for Ubuntu 16.04 you can execute the following commands:
## You need root permissions sudo add-apt-repository ppa:webupd8team/java sudo apt-get update sudo apt-get install oracle-java8-installer
To install Java manually you can download the latest Java SDK for Linux x64 file (linux-x64.tar.gz) from Oracle at http://www.oracle.com/technetwork/es/java/javase/downloads/index.html
Then you have to uncompress it , move it to an installation folder such as /opt and create a symbolic link:
## You can uncompress from the Download directory tar -zxvf jdk-8u91-linux-x64.tar.gz ## You need root permissions for the following sudo mv jdk1.8.0_91 /opt sudo ln -s /opt/jdk1.8.0_91 java ## Then you should have something like this: lrwxrwxrwx 1 root root 11 Jun 13 2016 java -> jdk1.8.0_91/ drwxr-xr-x 8 imedina imedina 4.0K Jan 6 13:31 jdk1.8.0_91/
and finally you must set the JAVA_HOME variable in the system or user bashrc file:
## Add this to ~/.bashrc or /etc/bash.bashrc export JAVA_HOME="/opt/java" PATH="$JAVA_HOME/bin:$M2:$PATH"
Independently of the installation procedure followed above you can check is Java properly installed by executing java -version, you should get something like this:
java version "1.8.0_91" Java(TM) SE Runtime Environment (build 1.8.0_91-b14) Java HotSpot(TM) 64-Bit Server VM (build 25.91-b14, mixed mode)
OpenCGA needs Tomcat 8 to run RESTful web services, we recommend to use Apache Tomcat 8.x since is the one we use in production but we have also used Jetty with any problem. You can install Apache Tomcat in Linux either via package manager or manually.
Installing via package manager can be more or less easy to do depending on the Linux distribution you use, keep in mind that Tomcat should run with the same user that OpenCGA. We will assume that OpenCGA will be installed with user opencga. Run the following to set Tomcat to run with user opencga. for example for Ubuntu 16.04 you can execute the following command:
## You need root permissions sudo apt-get install tomcat8 ## You can check that Tomcat is running by executing sudo service tomcat8 status ## Run the following to set Tomcat to run with user opencga cd /var/lib/tomcat8/ sudo service tomcat8 stop sudo chown -RL opencga:opencga ./* ## Edit the files /etc/init.d/tomcat8 and /etc/default/tomcat8 TOMCAT8_USER=opencga TOMCAT8_GROUP=opencga ## Then reload the service and restart sudo systemctl daemon-reload sudo service tomcat8 start
You can install Tomcat manually by downloading it from http://tomcat.apache.org/download-80.cgi and executing as the OpenCGA installation user, execute the following commands:
## You can uncompress from the Download directory tar -zxvf apache-tomcat-8.5.3.tar.gz ## You need root permissions to move to /opt and create a symbolic link sudo mv apache-tomcat-8.5.3 /opt sudo ln -s /opt/apache-tomcat-8.5.3 tomcat ## You can start and stop Tomcat executing /opt/tomcat/bin/startup.sh /opt/tomcat/bin/shutdown.sh
We recommend to make same changes in the Tomcat configuration:
- Increase Tomcat memory: you can do this by adding JAVA_OPTS=" $JAVA_OPTS -Xms1024m -Xmx12288m " to file bin/catalina.sh. In this example we have increased the memory to 12GB.
- Enable compression to save bandwidth: you can ask Tomcat to compress the HTML and JSON output, go to conf/server.xml and look for the connector 8080 and leave it like this:
<Connector port="8080" protocol="HTTP/1.1"
OpenCGA uses MongoDB 3.2.x+ to store Catalog database and also as a possible backened for the Variant Storage engine. The best ways of installing MongoDB is from the Linux package managers, you can follow MongoDB tutorials for Ubuntu and RedHat/CentOS at:
- Ubuntu: https://docs.mongodb.com/manual/tutorial/install-mongodb-on-ubuntu/
- RedHat/CentOS: https://docs.mongodb.com/manual/tutorial/install-mongodb-on-red-hat/
You can customise server configuration at /etc/mongodb.conf or the client at ~/.mongorc.js. Some recommended configuration for MongoDB:
- Always use WiredTiger engine
- Make sure the journal is enabled
- Make sure to have one directory per db with: directoryperdb=true
- For cluster installation we recommend to set up a Replica Set, this will improve performance and will make the installation more robust. OpenCGA versions 2.x+ utilise MongoDB's transactions, therefore a Replica Set is required.
Apache Solr 6.x is used in OpenCGA as a complementary search engine for improving the performance of some queries and aggregations, full text search and faceted queries to Variant database. Solr is highly reliable, scalable and fault tolerant NoSQL database, it provides distributed indexing, replication, load-balanced querying, automated fail over, recovery, centralised configuration and more. You can learn how to install Solr at https://cwiki.apache.org/confluence/display/solr/Installing+Solr.
OpenCGA needs to be able to create new Solr collections and provide the Variant search schema. To do this you need to download the OpenCGAConfSet-1.4.0.tar.gz, then you just need to uncompress it and copy it into your Solr folder installation (i.e., $SOLR_HOME/server/solr/configsets) ., for next version 1.4.0 you must use
- For Solr standalone installation, you have to copy the OpenCGAConfSet-1.4.0 configuration into the folder server/solr/configsets for Solr standalone installation. Now, you can create new core or collections dynamically from Solr command line or from REST web services, e.g.:
- For Solr cloud installation, you have to upload the OpenCGAConfSet configuation to your cluster by running the Solr zookeeper command line. In the following example command line, the OpenCGAConfSet configuration is located in the folder server/solr/configsets:
./bin/solr zk upconfig -n OpenCGAConfSet-1.4.0 -d server/solr/configsets/OpenCGAConfSet-1.4.0 -z localhost:9983
Now we can create collections from our search command line or from REST services, e.g.:
OpenCGA uses the bamCoverage tool to extract a bigwig from every alignment file that is indexed through our system. This tool can be found inside the DeepTools suite, and could be easily installed using pip, the package installer for Python.
pip install deeptools
For more information: https://deeptools.readthedocs.io/en/develop/content/installation.html
OpenCGA uses Apache Maven 3.x as building tool, we use maven to compile, build, install and run tests of OpenCGA, so you do not need Maven unless you want to compile and build the source code for any reason, you can follow Installation Guide > Building from Source Code instructions to learn how to do it. Remember that you can always get stable OpenCGA binaries from Installation#GettingOpenCGA.
Maven can be easily installed in Linux via package manager, you can execute with sudo or as root the following commands in Ubuntu or CentOS:
## Ubuntu 16.04 sudo apt-get install maven ## CentOS 7.x sudo yum install maven
Or you can also install Maven manually following this tutorial http://maven.apache.org/install.html
You can check that Maven is installed by executing mvn -v or mvn -version, you should get something like this:
## mvn -v or mvn -version Apache Maven 3.3.9 (bb52d8502b132ec0a5a3f4c09453c07478323dc5; 2015-11-10T16:41:47+00:00) Maven home: /opt/maven Java version: 1.8.0_91, vendor: Oracle Corporation Java home: /opt/jdk1.8.0_91/jre Default locale: en_GB, platform encoding: UTF-8 OS name: "linux", version: "4.4.0-57-generic", arch: "amd64", family: "unix"
You need to add a Maven Profile to set up some variables that will be injected during the building, you can learn more about this at Installation Guide > Building from Source Code.
OpenCGA uses Redis 2.8+ to cache queries to data. You can easily install Redis via package manager:
## Ubuntu 16.04 sudo apt-get install redis-server redis-tools ## CentOS 7.x sudo yum install redis
This is a new feature in OpenCGA and at the moment we use the default Redis configuration.
This is an optional backend plugin for OpenCGA storage. We have used both Hortonworks and Cloudera as Hadoop distribution. At the moment we recommend Hortonwork 2.5+.
OpenCGA uses HAProxy 1.5+ in the cluster installation to balance all REST web services call to the different deployed Tomcats. You can easily install HAProxy via package manager:
## Ubuntu 16.04 sudo apt-get install haproxy ## CentOS 7.x sudo yum install haproxy
Here you can find an easy example for a very simple configuration, go to HAProxy for more documentation:
frontend http-in bind *:80 ## Asign 'webservices' to paths starting with 'opencga' acl webservices path_beg -i /opencga ## Redirect ACL 'webservices' to backend 'tomcat_prod' use_backend tomcat_prod if webservices ## backend definition balancing two Tomcat instances backend tomcat_prod cookie SERVERID insert nocache indirect balance roundrobin option httpclose option httpchk HEAD / server webprod01 10.21.2.3:8080 cookie webprod01tc check port 8080 inter 5s rise 2 fall 3 maxconn 300 server webprod02 10.21.2.5:8080 cookie webprod02tc check port 8080 inter 5s rise 2 fall 3 maxconn 300
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Remember OpenCGA Architecture: